Composite

Part:BBa_K077003:Design

Designed by: Floor Hugenholtz   Group: iGEM08_Groningen   (2008-07-15)


Hybrid promoter (cI and luxR regulated) and LuxI and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 760
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1436


Design Notes

Ligation of parts BBa_R0065(SpeI and PstI) and BBa_J37034(XbaI and PstI). Plasmid is pSB1A2


Source

BBa_R0065 and BBa_J37034

References